Effect of nuclear IL-33 on gene expression

The aim of this study was to examine the transcriptional regulatory activity of nuclear IL-33 independent of its well-described extracellular cytokine activity mediated by its receptor, ST2. Overall design: Single-cell clones of Human TE-7 esophageal epithelial cells, which lack expression of the ST2 receptor, and with lentiviral-mediated, Dox-inducible overexpression of full-length IL-33, a non-chromatin binding form of IL-33 (amino acids 112-270), or empty vector (pINDUCER20) were treated with 100 ng/mL of doxycycline (Clontech) or control media for 48 hours. Expression of wild-type and truncated IL-33 was verified by Western blot. RNA was isolated using Tripure reagent and subjected to genome-wide RNA-sequencing through the Cincinnati Children's Hospital Medical Center Gene Expression Core.