Investigation of the effect of 1,25(OH)2D3 on murine CD11+ dendritic cells. Investigation of the effect of 1,25(OH)2D3 on murine CD11+ dendritic cells

Gene expression was compared between CD11c+ bone marrow derived dendritic cells (BMDC) was compared between cells conditioned in 20nM 1,25(OH)2D3 (Vitamin D) or vehicle. A second analysis compared gene expression in 1,25(OH)2D3 or vehicle CD11c+ BMDCs which were matured in presence of 0.1ug/ml LPS. Overall design: Murine bone marrow was harvested and cells grown for 9 days in presence of GM-CSF and either 20nM 1,25(OH)2D3 or vehicle. CD11c+ were isolated by FACS after which RNA was extracted. In some experiments the CD11c+ cells were stimulated with 0.1ug/ml LPS for an additional 18hours