five

Abelmoschus esculentus L SMRT Raw sequence reads

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https://www.ncbi.nlm.nih.gov/sra/SRP298425
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Moreover, a combination of third- (PacBio) and second-generation (Illumina) sequencing technologies was applied to sequence full-length transcriptomes of okra. In order to get as many transcripts as possible, total RNA was extracted from three types of okra tissue (roots, stems and leaves).The high-quality RNA of different tissues was mixed equally and then used for library construction. Libraries were sequenced on the PacBio Sequel platform. Standard Iso-Seq classification and clustering protocol was used to obtain the full transcript sequence.
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2022-01-31
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