Genome wide expression analysis of senescent BJ fibroblasts in the presence or absence of various SMAD2/3 regulating molecules

BJ fibroblasts were grown to replicative senescence, and were then treated with control or SMAD2/3 regulating ligands. RNA was then harvested for RNA-seq. Overall design: BJ fibroblasts were grown to replicative senescence. Cells were then given treatment with control or SMAD2/3 regulating ligands Activin A (100ng/ml), TGFB2 (1ng/ml), Inhibin (100ng/ml)+TGFB2 (1ng/ml), or Inhibin (100ng/ml). Experiments, excepting control treatment, were done in duplicates, and each treatment was compared with controls to determine a fold change in transcription for genes.