Differential gene expression between WT and ERRa-null hearts. Mus musculus

Total RNA was isolated from 3 WT and 3 ERRa null hearts and independent hybridizations were performed using MOE430 2.0 microarrays. Expression profiling was conducted to determine changes in gene expression in hearts lacking ERRa. The expression of genes involved in heart and muscle development, muscle contraction, lipid metabolism, OxPhos, protein metabolism and transcription were affected by the loss of ERRa. Keywords: microarray and genetic modification Overall design: 3 hearts from WT and 3 hearts from ERRa-null mice were used in the study. The expression of genes in the ERRa KO hearts were compared to the reference WT hearts.