Modeling myocardial ischemia/reperfusion injury with human ventricular cardiac organoids

Current therapeutic drug exploring targeting at myocardial ischemia/reperfusion (I/R) injury is limited due to the lack of humanized cardiac models that resemble myocardial damage. Herein, we have developed cardiac organoids with ventricular cavity from human induced pluripotent stem cells (hiPSCs) and simulated typical I/R injury by hypoxia/reoxygenation (H/R).To investigate the mechanism of H/R-induced injury for human ventricular cardiac organoids, we set up the control group without any treatment and the H/R group with 24 h hypoxia followed by 24 h reoxygenation. Each group consisted of three samples, and RNA from each sample was extracted from the same batch of 8-16 independent cardiac organoids at day 21. Cardiac organoids were all differentiated from the hiPSCs line ZB11AOF.