Fast and highly sensitive full-length single-cell RNA-sequencing using FLASH-seq

Plate-based single-cell RNA-sequencing methods provide high transcriptome coverage and full-length mRNA information but have limited throughput. FLASH-seq (FS) is a full-length scRNA-seq method with increased sensitivity and reduced hands-on time compared to Smart-Seq3 (Hagemann-Jensen et al, 2020). The entire FS protocol can be performed in ~4.5 hours, is simple to automate and can be easily miniaturized to decrease resource consumption.