Mechanomics RT-DC Set 03 - HSPCs

Entry is a part of Mechanomics collection (10.6084/m9.figshare.c.5399826) that contains materials supporting findings presented in the following manuscript:
Urbanska, M., Ge, Y. et al.,
De novo identification of universal cell mechanics gene signatures
BiorXiv: https://doi.org/10.1101/2021.04.26.441418; first published: 27th April 2021, last updated: 4th Nov 2024
eLife: https://doi.org/10.7554/eLife.87930; first published: 23rd March 2023, last updated: 27th Dec 2024

This dataset contains mechanical measurement of CD34-positive human hematopoietic stem and progenitor cells (HSPCs) isolated from mobilized peripheral blood and treated (Arulmozhivarman et al. 2017 Sci Rep):
i) with a histone deacetylase inhibitor, valproic acid (VPA)
ii) untreated control (PBS).

Of note: This dataset was removed from later versions of the manuscript.
Measurements were performed using real-time deformability cytometry (RT-DC).
_rtdc.zip - contains data acquired during the measurments in '*.rtdc' format
_tsv.zip - contains extended data in a '*.tsv' format
_glmm.zip - contains result summaries of statistical analysis comparing Young's moduli of the different cell types using generalised linear mixed effects models

Arulmozhivarman, G. et al. Zebrafish In-Vivo Screening for Compounds Amplifying Hematopoietic Stem and Progenitor Cells: Preclinical Validation in Human CD34+ Stem and Progenitor Cells. Sci Rep 7, 12084 (2017). https://doi.org/10.1038/s41598-017-12360-0