Transcriptome analysis of pluripotent stem cells and fibroblasts.. Transcriptome analysis of pluripotent stem cells and fibroblasts.

Purpose: The purpose of this data is to compare the transcriptome of embryonic stem cell (ESCs), Induced pluripotent stem cell (iPSCs) and fibroblasts using RNA-seq. Methods: Total RNA was harvested by Trizol (Invitrogen) and RNA profiles of samples were generated by sequencing. Illumina Casava 1.8 software used for basecalling. Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence (using fastx_trimmer) then mapped to hg19 (genome build) whole genome using Tophat. This study provides a framework for the application of pluripotent stem cells RNA expression dynamics compare to fibroblasts profiling toward characterization. Overall design: Total RNA extracted from 3 human cell line samples representing hESCs, hiPSCs and fibroblasts.