What goes in must come out? The metabolic profile of plants and caterpillars, frass, and adults of Asota (Erebidae: Aganainae) feeding on Ficus (Moraceae) in New Guinea

Insect herbivores have evolved a broad spectrum of adaptations in response to the diversity of chemical defences employed  by plants. Here we focus on two species of New Guinean Asota and determine how these specialist moths deal with the leaf alkaloids of their fig (Ficus) hosts. As each focal Asota species is restricted to one of three chemically distinct species of Ficus, we also test whether these specialized interactions lead to similar alkaloid profiles in both Asota species. We reared Asota caterpillars on their respective Ficus hosts in natural conditions and analyzed the alkaloid profiles of leaf, frass, caterpillar, and adult moth samples using UHPLC–MS/MS analyses. We identified 43 alkaloids in our samples. Leaf alkaloids showed various fates. Some were excreted in frass or found in caterpillars and adult moths. We also found two apparently novel indole alkaloids likely synthesized de novo by the moths or their microbiota—in both caterpillar and adult tissue but  not in leave..., Please refer to Fontanilla et al. (2022) for detailed methods. These data were collected from plant and insect tissue in New Guinea at the Binatang Research Centre and analysed using UHPLC-MS/MS at the University of Turku., , # What Goes in Must Come Out? The Metabolic Profile of Plants and Caterpillars, Frass, And Adults of Asota (Erebidae: Aganainae) Feeding on Ficus (Moraceae) in New Guinea

[https://doi.org/10.5061/dryad.tmpg4f553](https://doi.org/10.5061/dryad.tmpg4f553)

These data comprise 13 files and one annotated R script entitled 'Asota MS Analysis R 250923'. These data report the alkaloidal compounds extracted from Asota moths (Erebidae: Aganainae). We studied two species of Asota (A. eusemioides and A. heliconia) across three species of Ficus (F. septica, F. pachyrrhachis, and F. hispidioides) in a rearing experiment outlined in detail in Fontanilla et al. (2022). We collected moths at different developmental stages (caterpillars and adults) as well as their frass and leaves from their host plants. All samples were analysed using UHPLC-MS/MS and in total we found 43 alkaloidal compounds and using multivariate data analysis we explored the occurrence of compounds across sample types (including ac...