RNA sequencing of rhabdomyosarcoma RD cells transfected with NRAS siRNAs for 48 h or treated with 300nM ERKi SCH772984 for 24 h

To compare gene transcription changes induced by NRAS knockdown or ERK inhibition, we performed RNA-seq analyses in the NRAS Q61H RD cells after 48 h NRAS suppression by 2 independent NRAS siRNAs, or 24 hours after ERK inhibitor treatment (ERKi). RD cells were transfected with nonspecific (NS) or two independent NRAS siRNAs (NRAS1 and NRAS2) for 48 h or treated with DMSO or 300nM ERK inhibitor SCH772984 for 24 h. Each condition was done in duplicates.