Comparative genomic hybridization of human embryonic stem cell lines deposited in the National Stem Cell Bank

The Purpose of this series of experiments is to identify copy number variations, duplications, and deletions in human embryonic stem (hES) cell lines deposited to the National Stem Cell bank and reveal the difference between different hES cell lines. CGH can achieve these aims at higher resolution. Keywords: comparative genomic hybridization Genomic DNA of different human embryonic stem cell lines deposited to the National Stem Cell Bank are extracted and hybridized to NimbleGen CGH microarray, using genomic DNA of hESC H1 or H9 as common reference. Difference between different stem cell lines will be revealed.