Isotype-specific plasma cells express divergent transcriptional programs

We utilized single cell-indexed custom RNA-sequencing to interogate the transcriptomes of isotype specific plasma cells at homeostasis and following immunization with a model antigen Overall design: Murine plasma cells were sorted as (Gr1-, CD3-, IgD-, CD138+) with additional sorting gates by isotype (either IgG1, IgG2b, IgG2a, IgA or IgM). Antigen specific plasma cells (NP+ Lambda1+) were isolated from draining lymph nodes before and 5 and 14 days after primary immunization with NP-KLH. Steady steady plasma cells were isolated from the spleen.