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Tuxedo bug report: H. saltator simulated data

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Figshare2015-02-03 更新2026-04-29 收录
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I randomly selected a 150kb region from Harpegnathos saltator to create a demo data set for instruction. I extracted sequences for the transcripts encoded in the region and used wgsim to simulate Illumina reads for these transcripts. The idea was to use the Tuxedo workflow (Bowtie + Tophat + Cufflinks) to map the reads back to the genome, reconstruct transcripts, and do differential expression analysis. Unfortunately the latest version of Cufflinks (as of Feb 3 2015) fails for this data set, crashing with a segfault and without any helpful error message.

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2015-02-03
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