Transcriptome-wide analysis links the short-term expression of the b isoforms of T-cell intracellular antigens to protective proteostasis-mediated survival and quiescence

Control of gene expression depends on genetics and environmental factors. The T-cell intracellular antigens T-cell intracellular antigen 1 (TIA1), TIA1-like/related protein (TIAL1/TIAR) and human antigen R (HuR/ELAVL1) are RNA-binding proteins that play crucial roles in regulating gene expression in both situations. This study used massive sequencing analysis to uncover molecular and functional mechanisms resulting from the short-time expression of the b isoforms of TIA1 and TIAR and HuR in HEK293 cells. Three comparisons (altered vs control) were carried-out using three biological replicates per sample type. RNA-seq was performed with 100 nt strand-specific and paired-ends reads. Illumina HiSeq 2000 sequencer was used.