Fine scale time-series RNA-Seq of shoot and root responses to Nitrogen supply

Arabidopsis plants grown in a hydroponic media with sufficient Nitrogen were given a 60mM supply of Nitrogen. Triplicate sets of plants were sampled at time points 0,5,10,15,20,30,45,60,90 and 120 minutes after supply from treated and control plants (20mM KCl). The samples were flash frozen in liquid N and used to construct RNA-Seq libraries to assay transcriptional changes in the shoots and roots, separately, in response to Nitrogen. Overall design: Time series of shoot and root mRNA profiles from Root and shoot were generated at 10 time points after Nirogen supply using Illumina HiSeq platform.