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Colocalization of YFP-Tagged Gag with Antibody-Detected Viral Proteins.

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https://figshare.com/articles/dataset/_Colocalization_of_YFP_Tagged_Gag_with_Antibody_Detected_Viral_Proteins_/493307
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Primary CD4+ T cells and P2 cells infected with VSV-G-pseudotyped HIV-1 encoding Gag-iYFP or Gag-YFP were cultured for two days, immunostained for viral proteins, and examined by fluorescence microscopy. YFP-positive polarized cells were examined for colocalization of the cell surface YFP-tagged Gag proteins and viral antigens detected by anti-p17MA or anti-gp120 (b12).
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2010-10-28
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