Colocalization of YFP-Tagged Gag with Antibody-Detected Viral Proteins.
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Primary CD4+ T cells and P2 cells infected with VSV-G-pseudotyped HIV-1 encoding Gag-iYFP or Gag-YFP were cultured for two days, immunostained for viral proteins, and examined by fluorescence microscopy. YFP-positive polarized cells were examined for colocalization of the cell surface YFP-tagged Gag proteins and viral antigens detected by anti-p17MA or anti-gp120 (b12).
创建时间：
2010-10-28
