Todiramphus RADseq data

We extracted genomic DNA from high-quality, ethanol-preserved frozen tissue samples using a manual magnetic bead-based protocol based on Rohland & Reich (2012) for samples from each of eight Todiramphus species (range = 3-35 individuals per species). Library preparation was performed at the University of Kansas Genomic Sequencing Core, following the protocol outlined in Manthey and Moyle (2015). Briefly, samples were digested with the enzyme NdeI, ligated with custom barcodes, size selected for fragments between 500-600 bp in length, PCR amplified, and bead purified. Libraries were then pooled across projects and sequenced across multiple flow cells on a NextSeq550 Illumina sequencing platform, using the high-output option to generate single-end 100 bp reads. These 83 samples all passed quality control thresholds (see: https://devonderaad.github.io/todiramphus.radseq/todi.filt.html) and were used in downstream analyses.