Guanidine hydrochloride (GdmCl) half concentration (C<sub>1/2</sub>) and melting temperature (T<sub>m</sub>) obtained for the EcRDBD<sub>WT</sub>, its mutants and the UspDBD in chemical and thermal denaturation experiments in comparison with the RosettaDesign scoring.
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The GdmCl concentration was taken for 50% of the protein fraction unfolded each and melting temperature values were assigned to the thermal denaturation curve derivative maxima (see Figure 6, Figure 7, insets and Figure 8). An asterisk sign for the EcRDBDWT score indicates value calculated for a side chain rotamers redesign structure (without any substitutions). The lower RosettaDesign score value the better structure [17].
创建时间：
2014-01-23
