Transcriptional profile of Mycobacterium tuberculosis in an in vitro model of intraocular tuberculosis

We studied M. tuberculosis transcriptome by whole genome microarray for the identification of key pathogenic determinants that are involved in the pathogenesis of intraocular tuberculosis. Retinal pigment epithelial (ARPE-19) monolayer cells were infected with M. tuberculosis H37Rv (MOI of 10:1). On 3-day post infection (dpi) intracellular M. tuberculosis RNA was isolated and compared to the control M. tuberculosis RNA isolated from inoculum used during infection. Three independent experiments were performed. Altogether, 2 control and 6 experimental samples were analyzed on 8 X 15K format microarray slides.