Circular RNA Profiling of Neutrophil Transcriptome Provides Insights into Asymptomatic Moyamoya Disease

Microarray based circRNAs profiling was determined between neutrophil samples from patients with asymptomatic MMD and healthy subjects. The microarray results were followingly confirmed by quantitative reverse-transcription PCR. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses (KEGG) were adopted for annotation and predicting the functions of differentially expressed circRNAs. Results From this comparative circRNA microarray analysis of neutrophil samples from patients with asymptomatic MMD and healthy subjects, 123 circRNAs were identified differentially expressed between the two groups. Of these, 54 were upregulated and 69 were downregulated compared to controls (fold change > 2.0 and P < 0.05). Between July,2014 and Novemember,2015, five patients diagnosed with asymptomatic moyamoya disease by HR-MRI in Xuanwu hospital and five matched healthy volunteers were enrolled in our microarry detection.Overall, three male patients and two female patients with MMD (age range, 45-58 years) were included in this research, which were compared with five age- and sex-matched healthy volunteers (age range, 45-58 years).