Exome sequence of MCAS-derived cancer cell lines that are sensitive and resistant to PI3K/mTOR inhibition (BEZ235, Torin1, Rapamycin). Homo sapiens

Treatment of the MCAS tumor cell line with BEZ235 in reconstituted basement membrane proteins (3D) induces a cytostatic state in which cells are able to survive but not proliferate. An MCAS-derived cell line, MCAS-R, which proliferates in the presence of BEZ235 as well as Torin1 and Rapamycin, was derived. Whole exome sequencing of MCAS-R and the sensitive control line, MCAS-S, was performed in an effort to identify mutations underlying resistance to PI3K/mTOR inhibition.