Single cell RNA sequencing of healthy human airway epithelia with IL-13 treatment time course. Single cell RNA sequencing of healthy human airway epithelia with IL-13 treatment time course

The goal of this study was to examine changes in cell composition and gene expression with IL-13 treatment over time within different cell types from air-liquid interface cultures from healthy primary human airway epithelia. Overall design: Well differentiated, primary human airway epithelia at the air liquid interface were dissociated and prepared for single cell sequencing via 10X Genomics Chromium controller, and sequenced on Illumina HiSeq4000