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Rat duodenum during immobilization

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE4426
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In the present study, we examined duodenal gene expression in rats subjected to immobilization, to elucidate the mechanism of the stress response in the duodenum. Keywords: stress response Total RNA was extracted from the duodenal mucosa with an acid guanidinium thiocyanate-phenol-chloroform mixture. Total RNA was analyzed using the rat genome U34A microarray (Affymetrix). Gene intensity information was converted to a mean intensity for each gene using the manufacturer’s proprietary software (version 4.0; Affymetrix), which includes routines for data filtering and normalization.
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2017-02-21
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