Transgene integration typically takes place in an easy-to-transform laboratory variety before the transformation event is introgressed through backcrosses to elite cultivars. As new traits are added t
Study aimed to achieve high levels of multiallelic targeted mutagenesis in sugarcane using CRISPR/Cas9 to target the chlorophyll synthesis gene magnesium chelatase.
Additional file 2. Sequence and location of three gRNAs in the lemon white1 gene. Description: Multiple sequence alignments of lemon white1 orthologs from sorghum and miscanthus and the three gRNAs us