DNA-seq of the spontaneous suppressor mutants of gip1 in Fusarium graminearum

Gip1 is a non-pheromone receptor that spatiotemporally regulates two distinct sexual differentiation processes during perithecial development in the wheat scab fungus Fusarium graminearum. Interestingly, on mating plates of the gip1 mutant older than two weeks, clusters of fertile perithecia with asci and ascospores were occasionally observed, suggesting the occurrence of spontaneous suppressor mutations. To identify the mutant genes, three suppressor strains, S1, S5, and S7, were selected for whole-genome sequencing analysis.