Figure A. Schematic of the conjugation strategy used to couple biotinylated molecules to MIS416.

Figure B. Schematic representation of conjugate A1. Figure C. Calibration curve of streptavidin-PE (excitation 500nm, emission 570nm). Figure D. Demonstration of linkage of NHS-biotin to MIS416. The graph shows the percentage of streptavidin attached to MIS416, calculated by dividing the total amount of fluorophore coupled to MIS416 over the total amount of fluorophore in the solution before the reaction. Error bars represent SEM. The experiment was repeated four times. Figure E. Calibration curve of biotin-SS-SIINFEKL-FAM and biotin-ttds-SIINFEKL-FAM (excitation 488nm, emission 520nm). Figure F. Evaluation of activation marker expression on DCs after treatment with conjugation A and B. Table A. Amount of SIINFEKL conjugated to MIS416 in conjugates A and B. Table B. Conjugates used in this study. (DOCX)