Pearson’s correlation coefficients of internal FVIII in GMVECs and HUVECs concurrently stained with VWF.

Pearson’s correlation coefficients (PCC) were measured in GMVEC and HUVEC images stained concurrently for FVIII and VWF. FVIII was detected using mouse anti- FVIII plus goat anti-mouse IgG AF-647 and VWF was detected with rabbit anti-VWF plus chicken anti-rabbit IgG AF-488. PCC values >0.5 signify detection correlation. [26] Data comparing FVIII and VWF were analyzed in 15–23 images from 4–5 experiments in GMVECs and in 27–29 images from 7 experiments in HUVECs.aIndicates signal correlation in the two channels. [26]Pearson’s correlation coefficients of internal FVIII in GMVECs and HUVECs concurrently stained with VWF.