SULT2B11b regulates sensitivity to TNF-mediated cell death in Prostate Cancer

Single-cell mRNA sequencing (scRNA-seq) study was conducted to compare the transcriptomes of SULT2B1b knockdown (KD) versus non-targeting (Control) KD LNCaP cells. Over 2,000 differentially expressed (DE) genes were identified along with alterations in numerous canonical pathways, including the death receptor signaling pathway. Overall design: To prepare for single-cell analysis, 2x10^5 LNCaP cells were plated in a 12-well plate coated with poly-L-lysine and allowed to adhere for 24 hours. LNCaP cells were transfected with non-targeting (control) siRNA (Dharmacon) or SULT2B1 siRNA (IDT, HSC.RNAI.N004605.12.2), previously validated to knockdown SULT2B1b expression and function in prostate cancer cells (Figure 3.4E).234 48 hours following siRNA transfection, cells were harvested with trypsin and stained with Zombie Violet viability dye (Biolegend, 423114) according to the manufacturer's instructions.