Sea-ATI Unravels Novel Vocabularies of Plant Active Cistrome. Arabidopsis thaliana

The cistrome consists of all cis-acting regulatory elements recognized by transcription factors (TFs). However, only a portion of the cistrome is active for TF binding in a specific tissue. Resolving the active cistrome in plants remains challenging. In this study, we report the assay sequential extraction assisted-active TF identification (sea-ATI), a low-input method that comprehensively profiles the DNA sequences recognized by TFs in a target tissue. We applied sea-ATI to seven plant tissues to survey their active cistrome. The generated sea-ATI libraries together with the ATAC-seq and RNA-seq data of the corresponding tissues are collected and uploaded.