Pseudomonas sp. Teo4 Genome sequencing and assembly. Pseudomonas sp. Teo4

Genetic and transcriptomic analysis of a bacterial degradation pathway for the buffer compound TRIS.TRIS (2-amino-2-hydroxymethyl-propane-1,3-diol) is a synthetic chemical that is widely used as a buffer, emulsifier, and pharmaceutical. TRIS was long considered biologically inert and no degradation pathway for TRIS was known. We isolated several Pseudomonas sp. strains that are able to metabolise TRIS as a carbon and nitrogen source. We sequenced the genomes of these isolates and used transcriptomic analyses to identify genes and proteins involved in the TRIS degradation pathway.