NR supplementation improves performance and mitochondrial respiration in some DGRP-TAZ mutants.

Day 10 average endurance (red, n = 10 vials, 200 flies), climbing speed (blue, n = 100 flies) and mitochondrial RCR (green, n = 3 biological replicates) with NR supplementation. Two-way ANOVA + Tukey. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. Despite the large separation of average RCR, the benefit provided to mitochondrial RCR by NR supplementation related to line 25208 showed a large degree of variation across repetitions in 2 of the 4 cohorts (25208 +NR and 25208 TAZ), therefore resulting in modest p-values or non-significant results: 25208 vs 25208 +NR: *P = 0.033, 25208 vs 25208 TAZ: P = 0.99, 25208 vs 25208 TAZ +NR: *P = 0.046, 25208 +NR vs 25208 TAZ: *P = 0.044, 25208 +NR vs 25208 TAZ +NR: P = 0.98, 25208 TAZ vs 25208 TAZ +NR: P = 0.062.
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