The Secondary Resistome of methicillin-resistant Staphylococcus aureus to beta-lactam Antibiotics

The transposon library of USA300 JE2 was constructed using a two-plasmid, temperature-sensitive transposition system: pMARGK2b, which has been cloned to include the Himar1 transposon and mariner mosaic ends, and pFA545, which contains a mariner transposase. This library contains more than 400,000 unique transposon insertions, with an average distance of approximately 6 bp between each insertion. Aliquots of this transposon mutant library were grown with and without a subinhibitory concentration of beta-lactam antibiotics (oxacillin or cefazolin). Raw data were generated using MiSeq V2 50 cycles