Overall expression of genes in CRYM overexpressed Prostate cancer cell line LNCaP. Homo sapiens

RNA sequencing was employed to profile the entire mRNA transcripts. Total RNA from LNCaP transfected with control vector or CRYM bearing vector was isolated. RNA and samples were subjected to the sequencing on illumina's HiSeq 4000 platform. RNA-seq revealed the expression of 58,540 transcripts and of 34,878 (59.6 %) genes were expressed in LNCaP cells. 3226 (9.25 %) genes were altered by CRYM and 2.72 % were upregulated and 6.53 % were downregulated. Overall design: Cells were in transiently transfected cells with CRYM vector vs control in duplicate. GFP expression was monitored and total RNA was isolated at 48 hours upon transfection.