pro-TRH mRNA expression in the brain of zebra finch, Taeniopygia guttata

We show expression of proTRH in the brain of Taeniopygia guttata. The figure shows: (A) Multiple sequence alignment of prepro-TRH from Xenopus laevis, Danio rerio, Homo sapiens, Rattus norvegicus, and T. guttata showing evolutionary conserved TRH sequences flanked by the basic amino acids. (*) identical residues, (:) residues with strongly similar properties, (.) residues with weakly similar properties. The shaded portions represent TRH progenitor sequences with a great degree of similarity with that of other vertebrate species. (B) qRT-PCR product of pro-TRH mRNA of T. guttata. (C) Relative pro-TRH mRNA levels in the olfactory bulb (OB), left (LT) and right (RT) telencephalon, hypothalamus (Hyp), cerebellum (CB), midbrain (MB), and hindbrain (HB) of T. guttata. The agarose gel shows pro-TRH mRNA expression relative to beta-actin in respective brain regions.本研究呈现了斑胸草雀（Taeniopygia guttata）脑组织内促甲状腺激素释放激素原（proTRH）的表达特征。本附图包含如下内容：(A) 非洲爪蟾（Xenopus laevis）、斑马鱼（Danio rerio）、智人（Homo sapiens）、褐家鼠（Rattus norvegicus）及斑胸草雀的前原促甲状腺激素释放激素原（prepro-TRH）多序列比对结果，显示进化保守的TRH序列侧翼带有碱性氨基酸。其中，(*) 代表残基完全一致，(:) 代表残基性质高度相似，(.) 代表残基性质微弱相似。阴影区域代表与其他脊椎动物物种同源性较高的TRH前体序列。(B) 斑胸草雀proTRH信使RNA（mRNA）的实时定量逆转录聚合酶链反应（qRT-PCR）产物。(C) 斑胸草雀嗅球（OB）、左侧（LT）与右侧（RT）端脑、下丘脑（Hyp）、小脑（CB）、中脑（MB）及后脑（HB）中的相对proTRH mRNA表达水平。琼脂糖凝胶图展示了各脑区中以β-肌动蛋白为内参的proTRH mRNA表达情况。