Gene expression in Drosophila adult midgut

Midgut of Drosophila melanogaster is a useful model to study related to stem cell, cell differentiation, innate immune system, tissue regeneration, epithelial cell death, infection, and commensal bacteria. For such studies gene expression profile gives valuable information which enable us to design proper experiments. Based on this background gene expression profile was determined by RNAseq analysis. In this project, Drosophila carrying MyoIA-Gal4 and Tub-Gal80ts genes was used. Flies were grown at 18 °C and fed with standard fly food. Females were isolated from males 1-3 days after eclosion. Then 14 days after the eclosion, flies were transferred to a vial with food containing Mifepristone (Sigma Aldrich, M8046) and grown at 29 °C for 7 days.