Mettl3-mediated m6A Methylation Determines the Size of Endocrine Progenitor Pool and Postnatal Islet Mass

To investigate the role of RNA methyltransferase-like 3 (Mettl3)-mediated m6A modification in pancreatic endocrine differentiation in early embryonic period. We then performed m6A MeRIP-seq (GenSeq®? m6A MeRIP Kit) at E15.5 Mettl3pKO pancreas group and E15.5 WT pancreas group Overall design: We generated transgenic mice lacking Mettl3 specifically in Pdx1+ pancreatic progenitor cells(Mettl3pKO) and dissect E15.5 Mettl3pKO and WT pancreata for m6A MeRIP-seq.