RNAi screen of C. elegans homologs of human genes differentially expressed in invasive cells.

List of human genes (first column) that changed their expression with a log2 fold change ≥1 after SOX9 overexpression, TGF-ß stimulation, under hypoxia or during NC cell migration as indicated by a +. The names of the C. elegans orthologs that were screened by RNAi for AC invasion defects are indicated in the sixth and seventh column. Pvl indicates a protruding vulva phenotype at the adult stage, which is characteristic of vulval morphogenesis or AC invasion defects. The frequencies of BM breaching defects or of delayed BM breaching (after the Pn.pxx stage) are indicated. RNAi of genes causing a BM breaching defect are highlighted in green, and the negative control RNAi with the empty vector in red. n refers to the number of animals scored. AC, anchor cell; BM, basement membrane; NC, neural crest. (XLSX)