Cardiac-specific developmental and epigenetic functions of Jarid2 during embryonic development

Jarid2 cooperates with PRC2 for H3K27me3 accumulation on a subset of Jarid2 target genes in the developing heart, which contributes to repress differentiation of other lineages such as neural differentiation, and to guide normal myocardial development. Jarid2 forms a functional complex with PRC2 to increase or maintain H3K27me3 levels on the specific promoters in the developing heart. Sonicated chromatin from 20 pooled E17.5 fixed hearts was immunoprecipitated using H3K27me3 antibody, followed by the reversal of cross-linking and DNA purification. Immuno-enriched DNA targets were amplified by whole genome amplification and fluorescently labeled, which were then hybridized onto the Roche NimbleGen 3X720K RefSeq promoter arrays and scanned with an Axon 4000B.