Data underlying the publication: Microscale 3D-Printed Biocompatible, Soft Silicone Nerve Cuff for the Mouse Vagus Nerve

This dataset includes measurements for the Jacob’s Working Curve of BioRes-Silicone and electrocardiogram (ECG) recordings from in-vivo heart rate modulation experiments. For the Jacob’s Working Curve, three types of data were collected: (1) power readings obtained at the beginning of the experiment using a power meter; (2) exposure time which was set via MATLAB code (Mathworks); and (3) thickness measurement taken manually with a digital caliper (Neiko) capable of measuring to two decimal places. The Jacob’s Working Curve enables the use of BioRes-Silicone on other digital light processing (DLP) 3D-printing systems. The ECG data were collected from an adult ChAT-Cre (homozygous) × ChR2 (homozygous) mouse to observe heart rate reduction in response to blue light stimulation of channelrhodopsin-2 (ChR2) in the vagus nerve. Recordings were acquired using a PowerLab 2/26 bioamplifier (ADInstruments) and processed in LabChart software (ADInstruments). The final .txt file was exported directly from LabChart. These data confirm the nerve cuff successfully stimulates the vagus nerve.

本数据集包含BioRes-硅胶（BioRes-Silicone）的雅各布工作曲线（Jacob’s Working Curve）测量数据，以及活体心率调控实验中的心电图（electrocardiogram, ECG）记录。针对雅各布工作曲线，共采集三类数据：(1) 实验起始阶段通过功率计获取的功率读数；(2) 由MATLAB代码（Mathworks公司）设置的曝光时长；(3) 由精度可达小数点后两位的Neiko数显卡尺手动测得的厚度值。雅各布工作曲线可支持将BioRes-硅胶应用于其他数字光处理（digital light processing, DLP）3D打印系统。本次心电图数据采集自成年ChAT-Cre（纯合子）×ChR2（纯合子）小鼠，用于观测蓝光刺激迷走神经内的通道视紫红质-2（channelrhodopsin-2, ChR2）时的心率降低效应。实验记录采用PowerLab 2/26生物放大器（ADInstruments公司）获取，并通过LabChart软件（ADInstruments公司）进行处理，最终的.txt文件直接从LabChart导出。本数据集相关数据证实，该神经袖套可成功实现迷走神经刺激。