A molecular roadmap of the aorta-gonad-mesonephros region reveals BMPER as a novel regulator of HSC maturation [AGM]

In the developing embryo, haematopoietic stem cells (HSCs) emerge from the aorta-gonad-mesonephros (AGM) region but the molecular regulation of this process is poorly understood. Recently, the progression from E9.5 to E10.5 and polarity along the dorso-ventral axis have been identified as clear demarcations of the supportive HSC niche. To identify novel secreted regulators of HSC maturation, we performed RNA-sequencing over these spatio-temporal transitions in the AGM region, and supportive OP9 cell line. RNA-sequencing profiles of the aorta-gonad-mesonephros region from E9.5 embryos and E10.5 embryos sub-dissected into dorsal (AoD), ventral (AoV) and urogenital ridges (UGR) and pooled from between 15 and 34 embryos in three separate experiments.在发育中的胚胎内，造血干细胞（haematopoietic stem cells, HSCs）源自主动脉-性腺-中肾（aorta-gonad-mesonephros, AGM）区，但该过程的分子调控机制至今尚不明确。近期研究发现，胚胎发育从E9.5至E10.5的进程以及沿背腹轴（dorso-ventral axis）的极性特征，是支持性造血干细胞龛的明确分界标志。为鉴定调控造血干细胞成熟的新型分泌型调控因子，我们针对AGM区与支持性OP9细胞系（OP9 cell line）的上述时空动态变化过程开展了RNA测序（RNA-sequencing）。本数据集包含来自E9.5及E10.5胚胎的AGM区的RNA测序谱：该区域被显微解剖分离为背侧主动脉（AoD）、腹侧主动脉（AoV）与尿生殖嵴（urogenital ridges, UGR）三个部分，每组样本均混合了15至34个胚胎的组织，并完成了3次独立重复实验。