Genome-wide map of viral transcription factor ORF34 binding in Kaposi's Sarcoma Herpesvirus (KSHV)

We report the mapping of the genome-wide binding sites of a central component of a viral transcription complex from KSHV. We find that ORF34 binds at several locations on the viral genome but observed no detectable binding to the host genome. A majority of binding sites on the viral genome were upstream of known transcription start sites of mapped genes. Overall design: Chromatin for immuno-precipitation was prepared from iSLK cells latently infected with HA-ORF34 KSHV and reactivated with doxycycline and sodium butyrate for 48 h.