Transcriptome sequencing (RNA-Seq) overcomes limitations of previously used RNA quantification methods and provides one experimental framework for both high-throughput characterization and quantificat
Note: 1. “+” refers to sense strands, “−” refers to anti-sense strands.2. “Non-splice reads” refers to reads for the entire sequence is mapped to one exon; “Splice reads” also called junction reads, r
*BWA-MEM algorithm.Comparison of alignment statistics and SNP concordance with dbSNP (v137) between JAGuaR, GSNAP, MapSplice2 and TopHat2. SNVs were identified with a minimum total coverage of 6 reads
Transcriptome sequencing (RNA-Seq) overcomes limitations of previously used RNA quantification methods and provides one experimental framework for both high-throughput characterization and quantificat