Single cell RNA sequencing data obtained from aortic specimens of Fbn1G234D/G234D mouse model

We generated a novel genetic mouse model of fatal aortic dissection by introducing a pathogenic variant of Fbn1 (Fbn1G234D/G234D), identified in a patient with familial aortic dissection, into mice using the CRISPR/Cas9 system. To investigate the cellular heterogeneity underlying the progression of aortic dissection, we performed single cell RNA sequencing on aortic tissues obtained from Fbn1G234D/G234D (AD) and wild-type mice (WT). Following anesthesia, aortic tissues—including the ascending aorta and aortic arch up to the left subclavian artery—were collected from 3- and 5-week-old wild-type (WT) and Fbn1G234D/G234D mutant (AD) mice. The tissues were enzymatically digested to generate single-cell suspensions, which were then processed using the Chromium™ Single Cell Platform (10x Genomics) according to the manufacturer’s protocol. A total of eight single-cell suspensions were prepared for the study. For 3-week-old mice, the first batch included pooled samples from WT (n=2) and AD (n=2), and the second batch included another set of pooled WT (n=2) and AD (n=2). Similarly, for 5-week-old mice, the first batch consisted of WT (n=2) and AD (n=2), and the second batch included WT (n=3) and AD (n=3).