Vitis vinifera Transcriptome or Gene expression. Vitis vinifera

This is a 24 hour time course experiment to investigate changes in gene expression in deseeded berries sampled every three hours within a 24 hour period at 2 time points during development i.e. T4 (preveraison) & T15 (postveraison). Changes in gene expression within the 24 hour period were analyzed to identify expression profiles that coincided with diurnal changes. The gene expression profiles were then to be compared between the two developmental time points T4 v T15). Keywords: Diurnal time course Overall design: Nine time points during the diurnal cycle were analyzed at preveraison and post veraison for gene expression changes using the Affymetrix one colour array platform. Two biological replicates were collected for each developmental stage. Biotin labeled probes were prepared from the extracted RNA from each of the pooled berry samples and hybridized to the Vitis vinifera GeneChip. The sample/GeneChip position on the Affymetrix hybridization station was randomized using the AlgDesign package from R.