Identification of TWIST1-associated genomic regions in human breast cancer cell MCF7

In this study, we aimed to characterize the stimulatory role of TWIST1 in breast cancer cell proliferation, EMT and metastasis, uncover the underlying molecular mechanism, and identify potential therapeutic targets for treating BLBC. RNA-Seq analysis were performed to identify differentially expressed genes influenced by TWIST1 overexpression in breast cancer cell MCF7. Pathway enrichment analysis and gene set enrichment analysis were performed to identify TWIST1-regulated signaling pathways, particually the cancer-associated pathways. ChIP-Seq analysis was further performed to identify TWIST1-associated genomic regions in MCF7-TWIST1 cells. The potential direct target genes of TWIST1 were identified by combined analysis of the gene expression profiling data and ChIP-Seq data. TWIST1-associated genomic regions in MCF7-TWIST1 cultured in DMEM complete medium were measured at 24 hours after cell passage. Two samples were analyzed by using FLAG antibody. Samples were used as a control and one overexpression sample.