Characterization of the intergenerational impact of in utero and postnatal oxycodone exposure. Characterization of the intergenerational impact of in utero and postnatal oxycodone exposure

Based on the significant physical attributes observed at P14 in the F1 and F2 generations exposed to oxycodone in utero (IUO) and postnatally (PNO), we performed RNA-Seq analysis on the nucleus accumbens (NAc). The NAc was chosen given its association with the reward pathway. RNA-Seq analysis showed several up-and down-regulated genes among the IUO, PNO, and saline groups in both the F1 and F2 generations Overall design: RNA was isolated from the nucleus accumbens (NAc) of P14 Sprague Dawley rats that were exposed to oxycodone in utero (IUO) or postnatally (PNO). Samples were taken from F1 and F2 generations, allowing for intergenerational comparison. Biological replicates (n=4) taken from separate litters were used for each group.