Comparison of expressed pdxJ variants on global gene expression in E. coli

DNA microarray analyses was carried out to identify genes which are differentially expressed in E. coli when mutant variants of pdxJ are expressed that rescue growth of auxotrophic E. coli delta serB and delta serC mutants. Cells were grown in minimal M9-glucose medium. When a culture reached an optical density of 0.5 cells were harvested by centrifugation for RNA preparation. For each comparison pdxJ(11+) / pdxJ(11-) and pdxJ(X3+)/ pdxJ(WT) two independent biological samples grown from separate cultures were used.