Yuting Sun, Yejing Ge, Jenny Dmevich, Yong Zhao, Mark Band, Jie Chen (2011) CIL:13599, Mus musculus, skeletal muscle cell. CIL. Dataset

Mouse hind limb tibialis anterior (TA) muscles were injected with BaCl2 followed by daily systemic administration of rapamycin (Rap) starting 7 days after injury. The animals were sacrificed at different days, and the injured muscles were dissected and cryosectioned followed by hematoxylin (blue) and eosin (pink to red) staining.

This image shows the regeneration of muscle tissue fourteen days after the injury with daily systemic administration of Rap from day 7 to day 13 and a single dose of adenovirus expressing follistatin was injected into the injured TA muscle on day 7. The growth inhibition of myofibers by Rap was rescued by recombinant follistatin expression. Myofibers were stained by eosin in red and nuclei were stained by hematoxylin in blue. This image corresponds to Fig 10B from JCB 189: 1157-1169, 2010. See also CIL: 13595, 13596, 13597, 13598, and 13600.

本研究中，对小鼠后肢胫骨前肌（TA）进行BaCl2注射，并于受伤后第7天开始每日进行雷帕霉素（Rap）的系统给药。在不同时间点处死动物，随后对受伤肌肉进行解剖和冰冻切片，并进行苏木精（蓝色）和伊红（粉红色至红色）染色。图像展示了在从第7天至第13天每日系统给药雷帕霉素后，受伤肌肉组织在第14天时的再生情况。在第7天对受伤的TA肌肉注射了表达 follistatin 的腺病毒单剂量。雷帕霉素对肌纤维的生长抑制作用通过重组 follistatin 的表达得到缓解。肌纤维被伊红染成红色，细胞核被苏木精染成蓝色。该图像对应于2010年《细胞生物学杂志》（JCB）第189卷第1157-1169页的图10B。参见CIL：13595，13596，13597，13598，以及13600。